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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
03/05/2005 |
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Data da última atualização: |
03/08/2018 |
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Autoria: |
PONTES, R. G. M. S. de; DIAS, D. G. S.; SILVA, S. F. da; MARTINS, E. S.; BERRY, C.; FALCAO, R.; GOMES, A. C. M. M.; PRACA, L. B.; SOARES, C. M. S. |
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Afiliação: |
ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen; Daniel Gerhein Souza Dias, CENARGEN; Silvania Ferreira da Silva, CENARGEN; Erica Soares Martins, CENARGEN; Colin Berry, Cardiff School of Biosciences/Cardiff University; ROSANA FALCAO, CNPAE; ANA CRISTINA MENESES M GOMES, Cenargen; LILIAN BOTELHO PRACA, Cenargen; Carlos Marcelo Silveira Soares, Bthek Biotecnologia Ltda. |
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Título: |
Screening of Bacillus thuringiensis strains effective against mosquitoes. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 2, p. 103-106, fev. 2005 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Prospecção de estirpes de Bacillus thuringiensis efetivas contra mosquitos. |
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Conteúdo: |
The objective of this work was to evaluate 210 Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective. These strains were isolated from different regions of Brazil and are stored in a Bacillus spp. collection at Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. The selected strains were characterized by morphological (microscopy), biochemical (SDS-PAGE 10%) and molecular (PCR) methods. Six B. thuringiensis strains were identified as mosquito-toxic after the selective bioassays. None of the strains produced the expected PCR products for detection of cry4, cry11 and cyt1A genes. These results indicate that the activity of mosquitocidal Brazilian strains are not related with Cry4, Cry11 or Cyt proteins, so they could be used as an alternative bioinsecticide against mosquitoes. |
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Palavras-Chave: |
bioinsecticide; bioinseticida. |
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Thesagro: |
Culex Quinquefasciatus. |
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Thesaurus Nal: |
Aedes aegypti. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/30508/1/40n02a01.pdf
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Marc: |
LEADER 01740naa a2200277 a 4500
001 1113847
005 2018-08-03
008 2005 bl uuuu u00u1 u #d
100 1 $aPONTES, R. G. M. S. de
245 $aScreening of Bacillus thuringiensis strains effective against mosquitoes.
260 $c2005
500 $aTítulo em português: Prospecção de estirpes de Bacillus thuringiensis efetivas contra mosquitos.
520 $aThe objective of this work was to evaluate 210 Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective. These strains were isolated from different regions of Brazil and are stored in a Bacillus spp. collection at Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. The selected strains were characterized by morphological (microscopy), biochemical (SDS-PAGE 10%) and molecular (PCR) methods. Six B. thuringiensis strains were identified as mosquito-toxic after the selective bioassays. None of the strains produced the expected PCR products for detection of cry4, cry11 and cyt1A genes. These results indicate that the activity of mosquitocidal Brazilian strains are not related with Cry4, Cry11 or Cyt proteins, so they could be used as an alternative bioinsecticide against mosquitoes.
650 $aAedes aegypti
650 $aCulex Quinquefasciatus
653 $abioinsecticide
653 $abioinseticida
700 1 $aDIAS, D. G. S.
700 1 $aSILVA, S. F. da
700 1 $aMARTINS, E. S.
700 1 $aBERRY, C.
700 1 $aFALCAO, R.
700 1 $aGOMES, A. C. M. M.
700 1 $aPRACA, L. B.
700 1 $aSOARES, C. M. S.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 40, n. 2, p. 103-106, fev. 2005
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
10/05/2022 |
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Data da última atualização: |
11/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LEAO, A. P.; FERREIRA FILHO, J. A.; PEREIRA, V. M.; ALVES, A. A.; SOUZA JUNIOR, M. T. |
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Afiliação: |
ANDRE PEREIRA LEAO, CNPAE; JAIRE ALVES FERREIRA FILHO, Universidade Federal de Lavras-UFLA; VALQUIRIA MARTINS PEREIRA, Universidade Federal de Lavras-UFLA; ALEXANDRE ALONSO ALVES, CNPAE; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Genomic Characterization of SNPs for Genetic Differentiation and Selection in Populations from the American Oil Palm [Elaeis oleifera (Kunth) Cortés] Germplasm Bank from Brazil. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Diversity, v. 14, n. 270, 2022. |
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DOI: |
https://doi.org/10.3390/d14040270 |
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Idioma: |
Inglês |
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Conteúdo: |
In this study, we used SNP markers to access the genetic components occurrence of genetic differentiation resulting from the selection processes applied to collect and maintain the germplasm bank of Elaeis oleifera (Kunth) Cortés from the Brazilian Amazon rainforest. A set of 1667 higher quality SNPs?derived from a previous GBS study?was used for genomic characterization and calculation of genetic parameters. There is differentiation in the distribution of alleles between populations for 78.52% of the tested loci. Genotypic diversity test results indicated strong evidence of genotypic differentiation between populations. Sixteen out of the nineteen tested deviated significantly from the expected allele frequencies in HWE, reinforcing the hypothesis that there was maybe a selection in the evaluated populations. A group of 568 loci with a higher probability of being under selection effects were selected, both directional and stabilizing. In total, 1546 and 1274 SNPs aligned in the genomes of E. oleifera and E. guineensis Jacq., respectively. These markers showed a wide distribution throughout the genome of the two species. In conclusion, the E. oleifera GB from the Brazilian Amazon rainforest has specific genetic structures and good genetic variability within populations. |
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Palavras-Chave: |
Conservation strategy; SNP markers. |
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Thesagro: |
Caiaué. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142814/1/Leao-et-al-2022.pdf
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Marc: |
LEADER 02024naa a2200217 a 4500
001 2142814
005 2022-05-11
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/d14040270$2DOI
100 1 $aLEAO, A. P.
245 $aGenomic Characterization of SNPs for Genetic Differentiation and Selection in Populations from the American Oil Palm [Elaeis oleifera (Kunth) Cortés] Germplasm Bank from Brazil.$h[electronic resource]
260 $c2022
520 $aIn this study, we used SNP markers to access the genetic components occurrence of genetic differentiation resulting from the selection processes applied to collect and maintain the germplasm bank of Elaeis oleifera (Kunth) Cortés from the Brazilian Amazon rainforest. A set of 1667 higher quality SNPs?derived from a previous GBS study?was used for genomic characterization and calculation of genetic parameters. There is differentiation in the distribution of alleles between populations for 78.52% of the tested loci. Genotypic diversity test results indicated strong evidence of genotypic differentiation between populations. Sixteen out of the nineteen tested deviated significantly from the expected allele frequencies in HWE, reinforcing the hypothesis that there was maybe a selection in the evaluated populations. A group of 568 loci with a higher probability of being under selection effects were selected, both directional and stabilizing. In total, 1546 and 1274 SNPs aligned in the genomes of E. oleifera and E. guineensis Jacq., respectively. These markers showed a wide distribution throughout the genome of the two species. In conclusion, the E. oleifera GB from the Brazilian Amazon rainforest has specific genetic structures and good genetic variability within populations.
650 $aCaiaué
653 $aConservation strategy
653 $aSNP markers
700 1 $aFERREIRA FILHO, J. A.
700 1 $aPEREIRA, V. M.
700 1 $aALVES, A. A.
700 1 $aSOUZA JUNIOR, M. T.
773 $tDiversity$gv. 14, n. 270, 2022.
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Embrapa Agroenergia (CNPAE) |
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